Mast cell activation test versus basophil activation test and related competing issues

Basophil activation test--brief comment and response.

Basophil activation test: handle with care.

The letter to the Editor by Dr Chirumbolo highlights the major pitfalls in basophil activation test (BAT) performance. In my view, basophil activation test should be considered as an additional clinical tool in the diagnosis of allergy and not the “absolute truth”. Basophils play a pivotal role as effector cells in allergic disease, despite representing a small population of peripheral leucocyt...

Basophil Activation Test for Chronic Urticaria

Dear Editor, Zehwan Kim et al, [1] presented a protocol to assay basophil activation by flow cytometry (FC) that included a phenotyping panel with CD123 and CD193 (CC chemokine receptor-3 [CCR3]). This method for the electronic capture of basophils in whole blood, never published before,raises some interesting questions. CCR3 (CD193), the eotaxin receptor, is commonly present in CD3-expressing ...

Basophil activation test is food adverse reactions

Methods 83 subjects (66/17 females/males) with reported food adverse reactions were included in the study. Eighteen atopic/allergic subjects were included as controls for the foods to which they had reported no reactions. BAT was performed on heparinazed blood incubated with food extracts. CD63 activation marker was evaluated on basophils by flow-cytometry. The stimulation index (SI) was calcul...

Basophil activation test in allergic rhinitis.

903 a different perception on rhinitis, be it caused by an IgEor a non -IgE -mediated challenge, because CD63 is easily activated by nonallergic stimulation.4 Furthermore, the ability to perform an accurate BAT depends also on the gating strategy used by the test to catch basophils in the flow cytometry electronic capture.5 The Flow2CASTTM (BÜHLMANN, Schönenbuch, Switzerland) used by Leśniak et...